human breast cancer cells mda-m231  (Korean Cell Line Bank)

Journal: Biotechnology and bioengineering

Article Title: Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads

doi: 10.1002/bit.25987

Figure Lengend Snippet: Representative phase contrast images of PLL- and chitosan-coated microcapsules with M231 cell growth over 7 days. M231 cells in single PLL-coated microcapsules (a) immediately after capsule formation, (b) 1 day after capsule formation, (c) 3 days after capsule formation, and (d) 7 days after capsule formation. M231 cells in single chitosan-coated microcapsules (e) immediately after capsule formation, (f) 1 day after capsule formation, (g) 3 days after capsule formation, and (h) 7 days after capsule formation. Scale bars are 200 μm.

Article Snippet: Cell Culture—MDA-MB-231 and Fibroblasts GFP-labeled MDA-MB-231-gfp (M231) human breast cancer cells (ATCC, Manassas, VA) were grown in standard cell culture conditions (37°C, 5% CO 2 , 95% RH) in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 2 mM l -glutamine.

Techniques:

Journal: Biotechnology and bioengineering

Article Title: Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads

doi: 10.1002/bit.25987

Figure Lengend Snippet: Viability comparison of M231 cells in chitosan- and PLL-shelled microcapsules, over a 5-day time course. (a) Bar graph depiction of mean values ± standard deviation, (b) image set of time course with corresponding mean viability (green indicates living cells, while red indicates dead cells).

Article Snippet: Cell Culture—MDA-MB-231 and Fibroblasts GFP-labeled MDA-MB-231-gfp (M231) human breast cancer cells (ATCC, Manassas, VA) were grown in standard cell culture conditions (37°C, 5% CO 2 , 95% RH) in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 2 mM l -glutamine.

Techniques: Comparison, Standard Deviation

Journal: Biotechnology and bioengineering

Article Title: Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads

doi: 10.1002/bit.25987

Figure Lengend Snippet: Co-cultured cells within continuous chitosan-shelled microstrands, with different prescribed spatial composition. Heterogeneous microstrands of RFP fibroblasts (red) and GFP M231 (green) cells were created with three different spatial configurations: (a–b) microstrand with fibroblasts in one half, and M231 cells in the other at (a) day 0 and (b) day 5, resulting in segregated cell populations with a single interface between the two cell types; (c–d) microstrand with RFP fibroblasts in the central third of strand, and M231 cells in either end, such that there are two fibroblast-M231 interfaces between the distinct cell types, (c) shown immediately after creation and (d) after 1 day of culture; and (e) a microstrand in which the both cell types were printed without spatial control of the individual cell types, resulting in a more random cell distribution within the strand. Scale bars are 200 μm.

Article Snippet: Cell Culture—MDA-MB-231 and Fibroblasts GFP-labeled MDA-MB-231-gfp (M231) human breast cancer cells (ATCC, Manassas, VA) were grown in standard cell culture conditions (37°C, 5% CO 2 , 95% RH) in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 2 mM l -glutamine.

Techniques: Cell Culture, Control

Journal: Biotechnology and bioengineering

Article Title: Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads

doi: 10.1002/bit.25987

Figure Lengend Snippet: Representative phase contrast images of PLL- and chitosan-coated microcapsules with M231 cell growth over 7 days. M231 cells in single PLL-coated microcapsules (a) immediately after capsule formation, (b) 1 day after capsule formation, (c) 3 days after capsule formation, and (d) 7 days after capsule formation. M231 cells in single chitosan-coated microcapsules (e) immediately after capsule formation, (f) 1 day after capsule formation, (g) 3 days after capsule formation, and (h) 7 days after capsule formation. Scale bars are 200 μm.

Article Snippet: GFP-labeled MDA-MB-231-gfp (M231) human breast cancer cells (ATCC, Manassas, VA) were grown in standard cell culture conditions (37°C, 5% CO 2 , 95% RH) in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 2 mM l -glutamine.

Techniques:

Journal: Biotechnology and bioengineering

Article Title: Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads

doi: 10.1002/bit.25987

Figure Lengend Snippet: Viability comparison of M231 cells in chitosan- and PLL-shelled microcapsules, over a 5-day time course. (a) Bar graph depiction of mean values ± standard deviation, (b) image set of time course with corresponding mean viability (green indicates living cells, while red indicates dead cells).

Article Snippet: GFP-labeled MDA-MB-231-gfp (M231) human breast cancer cells (ATCC, Manassas, VA) were grown in standard cell culture conditions (37°C, 5% CO 2 , 95% RH) in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 2 mM l -glutamine.

Techniques: Comparison, Standard Deviation

Journal: Biotechnology and bioengineering

Article Title: Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads

doi: 10.1002/bit.25987

Figure Lengend Snippet: Co-cultured cells within continuous chitosan-shelled microstrands, with different prescribed spatial composition. Heterogeneous microstrands of RFP fibroblasts (red) and GFP M231 (green) cells were created with three different spatial configurations: (a–b) microstrand with fibroblasts in one half, and M231 cells in the other at (a) day 0 and (b) day 5, resulting in segregated cell populations with a single interface between the two cell types; (c–d) microstrand with RFP fibroblasts in the central third of strand, and M231 cells in either end, such that there are two fibroblast-M231 interfaces between the distinct cell types, (c) shown immediately after creation and (d) after 1 day of culture; and (e) a microstrand in which the both cell types were printed without spatial control of the individual cell types, resulting in a more random cell distribution within the strand. Scale bars are 200 μm.

Article Snippet: GFP-labeled MDA-MB-231-gfp (M231) human breast cancer cells (ATCC, Manassas, VA) were grown in standard cell culture conditions (37°C, 5% CO 2 , 95% RH) in growth medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin/streptomycin, and 2 mM l -glutamine.

Techniques: Cell Culture, Control

Journal: Scientific Reports

Article Title: Programmed activation of cancer cell apoptosis: A tumor-targeted phototherapeutic topoisomerase I inhibitor

doi: 10.1038/srep29018

Figure Lengend Snippet: ( a–f ) Intracellular fluorescence intensity recorded for A549 cells 1 h after incubation with of PT-1 in the absence ( a–c ) of and ( d–f ) after irradiation with 405 nm light for 1 h over the area delineated by the yellow dashed line. ( g ) Cytotoxicity of PT-1 observed in two cancer cell lines (A549 and HeLa) and two human normal fibroblast cell lines (WI-38 and BJ) after photo-activation. ( h ) Cell viability (relative percentage) observed for the A549, HeLa, WI-38, and BJ cell lines after treatment with PT-1 (incubation for 1 h) followed by irradiation at 405 nm for 1 h. Viability was determined via a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay performed 24 h after photo-irradiation. *P < 0.05. ( i–l ) Phase contrast images of A549 cancer cells undergoing apoptosis. In these studies, the cells were incubated with 10 nM PT-1 for 30 min followed by irradiation with 405 nm laser light for 1 h over the area indicated by the yellow dashed lines ( j , l ).

Article Snippet: Twelve biotin receptor-positive cell lines; human lung carcinoma cells (A549), human cervical cancer cells (HeLa), human breast cancer cells (MCF7, MDA-M231), human liver cancer cells (HepG2, Huh7, Hep3B), human prostate cancer cells (Du145, PC3), human gastric cancer cells (NCI-N87, AGS), human pancreatic cancer cell (Panc-1), and a biotin receptor-negative cell: human normal embryonal kidney epithelial cell (293T) were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Fluorescence, Incubation, Irradiation, Activation Assay, MTT Assay